Charting the Unknown
by Teresa Gleason
He's the Indiana Jones of the parasitic world.
Scott Snyder, an associate professor of biology at the University of Nebraska at Omaha (UNO), is on a crusade of sorts. His evolutionary parasitology lab on the UNO campus is traversing the globe in search of new species to collect and classify. The goal, he says, is to determine the incredibly complex life cycles of these creatures and examine their evolutionary relationships through the use of molecular techniques.
His second-floor lab in Allwine Hall is an interesting amalgam of "old" and "new" basic science. On one bench, traditional dissecting microscopes are used to recover parasites from their animal hosts. Slides drying on nearby slide warmers await examination under compound microscopes that magnify specimens up to 1,000 times. On another bench, a thermocycler is used to amplify small amounts of DNA into millions of copies, which are then examined on special gels and processed through additional chemical reactions. Across from the thermocycler, an automated DNA sequencing machine decodes the processed DNA.
The lab's human inhabitants are part artisans, part historians and part geneticists. "We do everything from identifying new parasites via microscopy to DNA sequencing and manipulation," he says. "The use of genetic sequences is exciting because it allows us to examine relationships from the distant past and understand the current parasitic world in much more detail than we can using traditional methods alone."
Dr. Snyder is the recent recipient of a $295,000 grant from the National Science Foundation's Biodiversity and Inventories Program, which supports research into unknown aspects of the planet's biodiversity.
His work focuses on the parasites of Australian freshwater turtles, which currently represent five major divisions, or phyla. Of the 39 recognized species to date, 20 have been described since 1994. "Although this relative explosion in taxonomic activity would seem encouraging, in reality, it merely highlights how little we know about this fascinating fauna," he says.
In support of this theory, Dr. Snyder reels off the following statistics:
All 20 species described since 1994 are from the same phylum. In addition, these parasites represent only seven of Australia's 25 turtle species. Most were collected in or before 1994, and most were collected from southern Queensland or northern New South Wales. "In short, a brief period of activity that examined only one of the five phyla of turtle parasites in a restricted geographical area more than doubled the number of known species," he says.
In June of 2004, Dr. Snyder traveled to Australia to begin work on his theory that a large amount of turtle parasite diversity has yet to be uncovered. His examination of only two snake-necked turtles collected in the Mary River, Northern Territory revealed eight parasite species. Six of these species, Dr. Snyder says, are new to science, and at least one belongs in a novel genus – the taxonomic category ranking below a family and above a species.
During the same month, Dr. Snyder collected three Victoria River turtles from the Victoria River, Northern Territory. The trio hosted six species of parasites, five of which he classifies as new and two of which he says represent novel genera.
"My examination of a mere five turtles increased the total number of parasite species recovered from this region from one to 15, the number of parasites recovered from the snake-necked turtle from zero to 8, and the number of parasites recovered from the Victoria River turtles from zero to 6," he says.
The new National Science Foundation (NSF) funding will allow Dr. Snyder and his colleagues to travel the Australian continent to determine if parasite diversity in Australia is similar to that found in the United States. Both land masses are similar in size.
Dr. Snyder, who grew up in southeast Nebraska and graduated from Auburn High School, noted that collecting specimens in Australia poses a number of unusual challenges. First and foremost is the saltwater crocodile of northern Australia, which lives in rivers alongside the turtles that are the subject of his research.
"These crocs reach up to 18 feet in length and are happy to eat humans if given the chance," he said.
On land, Australia is home to more poisonous snakes than anywhere else in the world. Add this to the fact that Dr. Snyder works in incredibly remote areas, and the combination can be lethal if extreme care is not taken. "If you get bitten, there's no chance of making it to a hospital in time," he said. "It's a beautiful, uninhabited place, but its dangers make you change the way you do biology."
At the conclusion of the grant, Dr. Snyder and his colleagues - including Vasyl Tkach at the University of North Dakota – hope to describe as many as 100 new species. Tkach also received a grant for $185,000 from the NSF's Biodiversity and Inventories Program. These yet-to-be-discovered parasites, never before seen by another human being, will be returned to the UNO lab for microscopic examination and DNA analysis by Dr. Snyder and his student research assistants.
Natalie Black, a first-year student at the University of Nebraska Medical Center, said working in Dr. Snyder's UNO lab as an undergraduate helped her obtain scholarships that are putting her through medical school. "He pushed us to think for ourselves and to be scientists, not just students," Black said. "Working for him helped me gain an excitement for science and its practical applications."
Black said she valued Dr. Snyder's practice of taking his students to scientific meetings. "This was huge in helping us enjoy and be proud of the work we were doing and gave us another reason to want to work hard," she said.
Dr. Snyder also collaborates with a number of researchers from the United States and around the world to examine questions of parasite systematics, evolution and biodiversity. These collaborations all draw information from host and parasite life histories, DNA sequences, morphology and biogeography.
His work to date has shown that some of the turtle parasites he's discovered are related to schistosomes, parasites that infect more than 200 million people in tropical areas.
"At any given time, 2 billion people on this planet are infected with a parasite," Dr. Snyder said. "Of this number, 500 million are infected with malaria, which kills between one and two million people each year. By understanding the general pattern of parasitic evolution in a broad and diverse range of animals, the potential to better understand how parasites affect humans increases. Labs like ours are the first rung on that ladder of understanding."
Teresa Gleason is director of communications at UNO. She can be reached at firstname.lastname@example.org.
October 2012 > A Closer Look at IS&T's NUCIA
October 2012 > UNO Engages Students, Community for 2012 Elections
December 2011 > Conference Tags
September 2011 > Roskens Hall: An Educator's Dream
July 2011 > Arts and Sciences Hall: UNO's Cornerstone
April 2011 > UNOCCC: Twenty-Five Years of Caring
March 2011 > UNO's Libraries: A History
March 2011 > The Henningson Memorial Campanile
February 2011 > Mammel Hall
February 2011 > The Sapp Fieldhouse
January 2011 > Heydays of the Hayden House
December 2010 > UNO Faculty/Staff Earn WELCOM Light of Wellness Team Award
August 2010 > Anna S. Forman Commencement Remarks
May 2010 > John Treinen Commencement Remarks
March 2010 > My Ties and Cliff Hillegass
January 2010 > Megan Schuster Commencement Remarks
December 2009 > Man Killed by Pheasant
November 2009 > New Molecular Modeling Lab Key to Chemistry Research Work
September 2009 > Technology and Term Papers – A Photo Essay
October 2008 > Zero to 65 in 80 Semesters
September 2008 > H.RES.1372: Celebrating the 100th
September 2008 > UNO Researcher Spotlighted
March 2008 > Culture on Campus
February 2008 > In Her Own Words: Technology is crucial to spurring growth
December 2007 > Pacific Street Memories
November 2007 > WISE Women
October 2007 > Vincent Empowers Teachers, Students With Technology
August 2007 > Orientation Leader Represents UNO with Exuberance
August 2007 > In His Own Words: On Summer School
July 2007 > Changing Faces
June 2007 > MVHC @ 50
February 2007 > At Your Service
December 2006 > A Job Well Done
September 2006 > Australian Finches Aid UNO Professor's Research
July 2006 > Finding His Way
May 2006 > Calming the Anxious
April 2006 > The Kid's Doing All Right
March 2006 > Shot of a Lifetime
March 2006 > Going for Four
February 2006 > Standing Tall with Sierra
January 2006 > In-Your-Face Geology
January 2006 > Religion Meets Film
January 2006 > Finding a Home Port
December 2005 > Man of the Cloth
November 2005 > Volunteer Field Work
November 2005 > Charting the Unknown
October 2005 > Developing Business Overseas
October 2005 > After The Storm
September 2005 > Building Better Officers
September 2005 > Saving the Planet
August 2005 > Making the Abstract Tangible
August 2005 > Helping Neighborhoods Help Themselves
July 2005 > Improving Health through Technology
July 2005 > Reading the Signs
June 2005 > Small Steps to a Better Life
April 2005 > Honest Art
March 2005 > Sharing The Wealth
March 2005 > Engineering His Own Masterpiece
February 2005 > Native Daughter, Native Dreams
February 2005 > Puck Stops Here
January 2005 > Stressing the Familiar
January 2005 > Connecting the Community
January 2005 > Ticket to Cooperstown
December 2004 > Spirit of the Season
December 2004 > Nicholas Stergiou: Research in Motion
fall 2004 > Alzheimer's: Caring for the Caregiver's
fall 2004 > David Hawk: All In
fall 2004 > Anadelia Lamas: Planting Roots
fall 2004 > Dean Olson: Assessing the Threat
fall 2004 > Tom Warren: From the Chiefs to the Chief