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Endocrine BioServices Assay Laboratory - Equipment and Supplies
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Equipment and Supplies Inventory

BioTek ELx405 The Endocrine BioServices Laboratory has a number of specialized pieces of equipment to perform enzyme immunoassays (EIA) and radio immunoassays (RIA). Often times, this equipment is too expensive for researchers who do not regularly perform EIAs and RIAs, so as a core facility of the University of Nebraska system, our lab helps reduce the cost of research.

The following list is not meant to be an exhaustive inventory of the Endocrine BioServices Lab. Any special supplies that might be needed for a particular project can usually be ordered. Through our affiliation with suppliers, we receive substantial discounts on nearly all laboratory supplies.

Laboratory Equipment

  • Multiskan Ascent ® 96-well plate reader (filters: 405 nm, 390 nm, 490 nm)
  • BioTek ® ELx405 96-well plate washer
  • 1.5 - 2.0 mL microcentrifuges: 1 x 24 tube capacity, 2 x 12 tube capacity
  • Climate-controlled floor centrifuge w/ rotors for 36 centrifuge tubes or for 240 test tubes
  • 96-well plate shaker and incubator
  • Gamma counter - counts radioisotopes I125 and Co57 pipette
  • Scintillation counter (UNO core facility)
  • pH meter (pH 4.0 - 10.0)
  • Evap-o-racks (cool air stream evaporators)
  • Hot plates and hot water baths
  • Vortex mixers
  • Digital scales (to 0.0001 g)
  • Fume Hoods
  • Fridges (2 - 4 °C) for reagent, sample, and radioactive storage
  • Freezers (-20 °C) for reagent and sample storage

Pipettes

  • Eppendorf ® 12 and 8 channel pipettes, digital and standard: 5 - 100 μl and 20 - 300 μl
  • Eppendorf ® single channel pipettes: 10 - 100 μl and 100 - 1000 μl
  • Microman ® single channel pipettes: 5 - 25 μl, 20 - 50 μl, 50 - 250 μl
  • Eppendorf digital repeating pipettes: 5 μl - 10 ml

Standard Assay Supplies

  • Nunc ® 96-well immunoassay plates
  • Strip tubes and caps - 12 per row
  • Culture tubes/test tubes: 12 x 75 mm and 16 x 100 mm reagent
  • 1.5 ml microcentrifuge tubes
  • 15 ml centrifuge tubes
  • 10 ml salivettes (for saliva sample collection)
  • Weigh dishes and weigh paper
  • Beakers, graduated cylinders, Erlenmeyer flasks, and other glassware

Solvents and Reagents

  • Alcohols (ethanol and methanol)
  • Ethyl Acetate
  • Hexane
  • Ether
  • β-glucuronidase (for enzyme hydrolysis)

Please contact us for more information on pricing and lab availability.

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